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Sunday, September 25, 2016

Clinical Microbiology HW#4



Chapter 12
1. Seventeen patients in ten hospitals had cutaneous infections caused by Rhizopus. In all seventeen patients, Elastoplast bandages were placed over sterile gauze pads to cover wounds. Fourteen of the patients had surgical wounds, two had venous line insertion sites, and one had a bite wound. Lesions present when the bandages were removed ranged from vesiculopustular eruptions to ulcerations and skin necrosis requiring debridement. Fungi are more likely than bacteria to contaminate bandages because they
Can tolerate low-moisture conditions

2. In mid-December, a woman with insulin-dependent diabetes who had been on prednisone fell and received an abrasion on the dorsal side of her right hand. She was placed on penicillin. By the end of January, the ulcer had not healed, and she was referred to a plastic surgeon. On January 30, a swab of the wound was cultured at 35°C on blood agar. On the same day, a smear was made for Gram staining. The Gram stain showed large (10 µm) cells. Brownish, waxy colonies grew on the blood agar. Slide cultures set up on February 1 and incubated at 25°C showed septate hyphae and single conidia. The most likely cause of the infection is a
Dimorphic fungus

3. Ringworm is caused by a(n)
Fungus

4. A definitive host harbors which stage of a parasite?
Adult

5. The life cycle of the fish tapeworm is similar to that of the beef tapeworm. Which of the following is the most effective preventive measure?
Cooking fish before eating

Chapter 12 – Reading Questions
1. The term dermatophyte is typically used for fungi that cause which of the following?
Cutaneous mycoses

Chapter 13
1. Which of the following statements about viruses is FALSE?
Viruses use their own catabolic enzymes
**TRUE: viruses have genes; viruses contain DNA or RNA but never both; viruses contain a protein coat; viruses use the anabolic machinery of the cell

2. Dogs do not get measles because their cells lack the correct receptor sites for that virus.
TRUE

3. A clear area against a confluent "lawn" of bacteria is called a
Plaque

4. The definition of lysogeny is
Phage DNA is incorporated into host cell DNA

5. An envelope is acquired during which of the following steps?
Release

6. Most drugs that interfere with viral multiplication also interfere with host cell function.
TRUE

Microbiology Animation: Prions: Characteristics
1. The normal function of the PrP protein in mammals is believed to be:
assisting in normal synaptic development and function.


2. How do normal prion proteins (PrP) differ from the infectious prion proteins?
Normal PrP have alpha-helices; infectious PrP have beta-pleated sheets.

3. How does the number of infectious prions increase?
Prions transform normal proteins into the misfolded beta-pleated sheet configuration; therefore, prions multiply by conversion.

4. Why are the beta-pleated multimers of PrP potentially pathogenic?
The multimers are more stable and resistant to protease.

Chapter 12 – Reading Questions
1. Dinoflagellates are a type of unicellular __________. One genus of dinoflagellates is responsible for a foodborne disease called __________.
algae; paralytic shellfish poisoning (PSP)
**Dinoflagellates are a type of unicellular algae. During red tide, high levels of a neurotoxin produced by Alexandrium (a dinoflagellate) are found in shellfish. Consumption of these shellfish leads to a foodborne disease called paralytic shellfish poisoning.

Chapter 12
1. Which of the following arthropods does NOT transmit diseases by sucking blood from a human host?
Houseflies

2. Helminthic diseases are usually transmitted to humans by
Gastrointestinal route

3. The sporozoite, merozoite, gametocyte, and ring stages are all part of the Plasmodium life cycle
TRUE

4. Which of the following is the most effective control for malaria?
Eliminate Anopheles

5. Which of the following tends to be more complex in a parasitic helminth than in free-living helminths?
Reproductive system

What Does Old Age have to Do with it?
Barbara, a 68-year-old retired schoolteacher, was beside herself. Her husband Bob had passed away only a month ago, and a week later, her youngest daughter, 40-year-old Maria, had moved back in with her. Maria was in the process of divorcing her husband and was seeking sole custody of her three children. The children, ages 6, 8, and 12, were also living in the house with their grandmother. Barbara enjoyed playing games, gardening, and taking walks with her family, and on the whole did not mind having the extra company. The extra cooking and cleaning were taking a toll on her health, however. One morning, Barbara woke up with excruciating pain on her right rib cage. When she looked at her side in the bathroom mirror, she found that she had an extremely red rash with little vesicular lesions. The rash was localized to her right side and looked very similar to Image A. Concerned about the rash and the amount of pain associated with it, Barbara phoned her physician’s office and made an appointment for that afternoon.
During her appointment, Barbara discusses her current and previous medical history with Dr. Jones. Up until this rash appeared, Barbara had been a healthy individual. She is the appropriate weight for her height, and her blood counts have always been within normal ranges. As an adult, she rarely has gotten sick, even with minor illnesses such as colds. As a child, Barbara had received all of the appropriate vaccinations as scheduled and had been a relatively healthy young lady. In fact, Barbara could remember missing school for illnesses only twice during her entire school career: once when she had chickenpox in second grade, and once in high school when she had a severe case of the flu. Barbara’s medical history confirms Dr. Jones’s suspicion of a latent infection, so there is no need for sample collection.
After asking a few more questions about her medical history, Dr. Jones asks Barbara about her current living situation. Barbara mentions that her grandchildren are living with her. Dr. Jones asks about their general health. Barbara responds that the 6-year-old is getting over a cold, but that overall they are healthy individuals. Dr. Jones gives Barbara a prescription for acyclovir and tells her to come back if she has any further complications.

1. Which of the following best describes Barbara’s condition?
Shingles caused by the herpes zoster virus
**As a child, Barbara was infected with the varicella virus and contracted chickenpox. Though she recovered from the chickenpox, the virus was able to enter the peripheral nerves and eventually the central nerve ganglion, where it persists as viral DNA hidden from her immune response. As a result of factors in her life, Barbara’s virus was reactivated and traveled along the peripheral nerves to the cutaneous sensory nerves of the skin. This new outbreak produces the rash characteristic of a shingles infection. The occurrence of shingles is 10% to 20% in individuals who have had chickenpox.

2. What is the correct sequence of events for the replication of a DNA virus?
            a. virions attach to the host cells

            b. viral DNA is released into the nucleus of the host cell

            c. enzymes required for multiplication of viral DNA are produced via transcription and translation

            d. a copy of the DNA is made

            e. capsid and other structural proteins are manufactured

            f. virions ae assembled to form complete viruses and are released from the host cell

3. Which of the following factors could have contributed to Barbara’s development of shingles?
Her daughter and grandchildren moving into her house shortly after the death of her husband
Her age, 68.
**Many factors can contribute to reactivation of a latent virus. Research has yet to narrow it down to one thing, but stress, old age, and a change in the host’s immune system have been linked to viral reactivation.

4. Barbara is worried about spending time with her grandchildren while being treated for shingles. Can her grandchildren contract chickenpox or shingles from spending time with their grandmother?
No. Because of their ages, the grandchildren have most likely been vaccinated against the chickenpox. They are also safe from contracting shingles because they are young.

5. Barbara is curious to know more about shingles and the herpes zoster virus that causes it, so she spends many hours researching the virus. During this process, Barbara learns that many diseases are caused by viruses in the family Herpesviridae. One of particular interest to Barbara is human herpesvirus-1, also known as herpes simplex virus 1. Surveys of the U.S. population show that about 90% of individuals have been infected with this virus.

What disease does the human herpesvirus-1 cause?
Cold sores or fever blisters

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